Effect of aqueous extract edible mushroom Ganoderma lucidum on pathogenic bacteria isolated from children's' tonsils

Abstract

The aim of the research is to find natural alternatives to antibiotics that have the ability to inhibit pathogenic bacteria by identifying the inhibition of a hot water extract of Ganoderma lucidum against bacteria isolated from children's' tonsils, which included Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus, pseudomonas aeruginosa and Escherichia coli. The hot aqueous extract showed a significant inhibitory effect against the growth of isolated bacteria at a concentration of 200 mg/ml, where the highest inhibition diameter was 21.33 ± 0.577 mm in S. aureus, while the lowest inhibition diameter was 13.67 ± 0.57 mm in P. aeraginosa at the same concentration. All types of bacteria showed resistance to a concentration of 12.5 mg / ml. The results show the difference in the inhibitory effectiveness with different concentrations. Effective antibacterial chemical compounds were identified in the aqueous extract of G. lucidum by Gas Chromatography-mass spectrometry (GC-MS), which showed the compound 7-Hexadecenoic acid, methyl ester, (z)- (30.52%), which is an unsaturated fatty acid, It has antibacterial activity.